Identification of Pseudomonas aeruginosa from post-traumatic wounds in Dhaka and their antimicrobial susceptibility profiles: a single-center cross-sectional study

Abstract

The purpose of the experimental study was to isolate the target organisms Pseudomonas aeruginosa and Staphylococcus aureus, from patients' wounds, which were generally recognized as post-traumatic wounds. All 45 patients were selected for post-traumatic wound cases, with 41 female and 4 male patients, and samples were taken from wound-infected areas. Next, the samples were cultured in two selective media cetrimide agar and mannitol salt agar (MSA) for these two specific organisms. After that, subculture was done on nutrient agar. Following this, DNA extraction was conducted in order to do the polymerase chain reaction (PCR) and agarose gel electrophoresis. Lastly, Kirby- Bauer Disc Diffusion method was used and CLSI guideline was followed for Antibiotic Susceptibility Test (AST). We found 18 isolates of Pseudomonas aeruginosa but no growth rate of the other targeted organism, Staphylococcus aureus, in 45 samples. Even though all the isolates from the patient group showed some percentage of resistance toward the antibiotics. Moreover, gram-negative isolates of the patient group exhibited high resistance to imipenem, gentamicin, cefepime, ampicillin and susceptible were seen against amikacin, meropenem, and ciprofloxacin. Furthermore, gram-negative Pseudomonas aeruginosa is mostly resistant to cephalosporins, aminoglycoside, and carbapenem group of antibiotics. The findings of this study highlight the significance of regular surveillance for patterns of antibiotic resistance in wound infections, specifically in cases of post-traumatic wounds.