Method optimization for isolation of Klebsiella Bacteriophage from soil samples

Abstract

In case of antibiotic-resistance bacteria, bacteriophages are widely used as a substitute for many antibiotics in the field of medical science. In this study, the objective was to segregate and point out bacteriophages against reference Klebsiella pneumoniae bacteria using four types of procedures. In addition, these procedures had been optimized to find a best possible outcome. Three types of soil samples were collected near the different hospitals, lakes, ponds, and the river from Old Dhaka (Boali), Tongi and Comilla. To isolate the bacteriophage plaque double layer agar plate method was used. This study can help to minimize costs, reliance on antibiotics and destroy antibiotic-resistant bacteria as the world is confronting a major encounter in managing various resistant strains.