Construction of vaccine against Herpes Zoster Virus Glycoprotein E using In-silico method

Abstract

Herpes zoster virus is a member of Alphaherpesvirinae subfamily and genus Varicellovirus of herpesviridae. Herpes zoster virus or Shingles is a viral disease. Recently, people are more suffering due to this virus. The varicella zoster virus (VZV), which also causes chickenpox, is what causes shingles. The initial virus infection in chickenpox, also known as varicella, often takes place during childhood or adolescence. There is only live attenuated vaccine for treating HZV and there are some antiviral drugs which are effective in early stage of this disease. But there is no promising in silico based vaccine for HZV. In this study, construction of herpes zoster vaccine through in silico method has been proposed against herpes zoster virus envelop glycoprotein E interferons. From UniProt database server Glycoprotein E sequence has been retrieved in FASTA file. Consequently, some online server are utilized to determine CTL, HTL and B-cell epitopes and other predictions. During study, a CTL , a HTL and a b-cell epitope shows best antigenic score and high inducing affinity with that herpes zoster glycoprotein E. The antigenicity rise happens from 0.51 to 0.53 when all antigenic epitopes are added in the construction. Z score, C-score ,GRAVY score are positive. The protein is hydrophilic which indicates it can be a good developed vaccine.