Determination of isolation & antibiotic susceptibility pattern of Salmonella spp. from fresh vegetables collected from local markets of Dhaka city

Abstract

Many outbreaks of food-borne Salmonella have been connected to raw vegetables; however, very little information is known on Salmonella contamination of raw vegetables in Dhaka's local wet markets. In this research, isolation of Salmonella isolates & their antibiotic susceptibility pattern were investigated. In total, 50 samples of raw vegetables were collected from the various wet markets from Dhaka city. The samples with the highest level of contamination were tomato (18%, n=10), green chili (18%, n=10), and mint (18%, n=10). The antibiotic resistance patterns of the 55 Salmonella isolates recovered from those positive samples. After analyzing their antibiotic resistance profile, it is found that 55 of those isolates are resistant to at least one or more class of antibiotics, indicating that they are resistant to multiple antibiotics. Antibiotic resistance and multi-resistance of Salmonella spp. have developed significantly during the past decade, particularly in the developing nations where the use of antibiotics for human and animal therapy has been increased. The rate of resistance observed for Penicilline and Metronidazole is (100%) higher than all others. Followed by Clindamycin (98%), Erythromycin (95%), Tetracycline (31%), Amoxicillin (48%), Streptomycin (10%), Chloramphenicol (15%), Ceftriaxone (11%). Gentamycin, Co–Trimoxazole, and Imipenem has shown lower resistance to this third generation antibiotics, rather than showing more susceptibility to Salmonella spp. (98%, 93%, and 78%). This research had found that the contamination of Salmonella in raw vegetables offers a potential risk to public health.